杨颖丽,施树倩,李嘉敏,张爱梅,张娅,李亚萍

• 1:西北师范大学生命科学学院

摘要(Abstract)：

以矮牵牛组培苗为材料,研究其在不同浓度Pb胁迫下的生长及光合生理反应.结果表明,Pb胁迫显著抑制矮牵牛组培苗的生长. 1和2 mmol/L Pb处理均导致叶绿素a、叶绿素b、类胡萝卜素和叶黄素含量显著下降,且2 mmol/L Pb胁迫导致的下降幅度更明显,但Pb胁迫不影响叶绿素a/b.随Pb处理浓度的增加叶绿素荧光参数光系统Ⅱ(PSⅡ)潜在活性、最大光化学效率、PSⅡ实际光化学效率、PSⅡ有效光化学效率、光化学猝灭系数以及光合电子传递效率逐渐减小,非光化学猝灭系数和调节性能量耗散电子产量增大, 0.05 mmol/L Pb处理不影响最大光化学效率、PSⅡ实际光化学效率和PSⅡ有效光化学效率.不同浓度Pb胁迫诱导矮牵牛组培苗叶片的气孔导度、净光合速率和蒸腾速率(T_r)显著下降,胞间CO_2浓度(C_i)明显增加. 0.05 mmol/L Pb处理不影响T_r和C_i, 0.8 mmol/L Pb处理使水分利用效率显著减小.徕卡正置荧光显微镜分析显示随Pb处理浓度的增加矮牵牛组培苗下表皮气孔密度显著增加,但气孔开度明显减小,保卫细胞形态发生畸变. Pb胁迫导致矮牵牛组培苗光合色素含量下降,光合系统捕获的能量减少,电子传递产额和光能转化利用效率降低,光合作用受阻,进一步导致生长受到抑制,且抑制效应具有浓度依赖性.

关键词(KeyWords)： 矮牵牛;铅胁迫;生长;光合特性

Abstract:

Keywords:

基金项目(Foundation): 甘肃省重点研发计划项目(20YF3FA043);; 兰州市人才创新创业项目(20-RC-84)

作者(Author): 杨颖丽,施树倩,李嘉敏,张爱梅,张娅,李亚萍

DOI: 10.13885/j.issn.0455-2059.2023.01.007

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